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CNN3 overexpression inactivates the PI3K/AKT signaling pathway. A549 and SK-MES-1 cells were transfected with NC or the CNN3 overexpression recombinant plasmid (CNN3-OV), and were referred to as NC and CNN3-OV, respectively. The levels of PI3K p85 α, p-AKT1 and AKT1 were examined by western blotting. ***P<0.001. CNN3, calponin 3; NC, empty vector pcDNA 3.1; p-, phosphorylated.
