Fig. 7.

Knockdown of Nox2 induces angiogenesis in Matrigel plugs in vivo. a. BALB/c nude mice were injected subcutaneously with 400 μL Matrigel mixed with 100 μL PVEC conditioned media (CM) for 15 days, then the plugs were excised. b. Body weight of BALB/c nude mice at day 1 and 15 in the trial. c. The Matrigel plugs were photographed (c), quantified for hemoglobin content (d), stained with CD31(e, upper) and hematoxylin and eosin (e, below). The CD31 immunofluorescence intensity (f) and cells (g) in the Matrigel plugs. Results are expressed as the mean ± SEM (n = 6).