Skip to main content
. Author manuscript; available in PMC: 2021 Jul 6.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2019 Jun 29;1866(10):1618–1633. doi: 10.1016/j.bbamcr.2019.06.015

Fig. 8.

Fig. 8.

SNAP-23 initial plasma membrane association is independent of Syntaxin 4. A–B. RBL mast cells were transfected with GFP Syntaxin 4 (STX-4) WT and GFP Syntaxin 4 ΔCT (STX-4 CT) mutant and plated on coverslip. After 6 h and 24 h of transfection their membrane association dynamics were observed under confocal microscope after PFA fixation. C. GFP Syntaxin 4 (STX-4) WT and GFP Syntaxin 4 ΔCT (STX-4 CT) transfected RBL cells were fixed with 4% PFA after 6 h and then counterstained with SNAP-23 specific antibody (red), separately. Then they were observed under confocal microscope. Scale bar is 10 μm. No significant staining was observed in the green or red channel when using an irrelevant Ab.