Table 3.
Hatchability and hatch residue variables at 21 d of incubation (doi) within in ovo treatment: diluent-injected control, and diluent containing 2.4 μg of vitamin D3 (D3) or 25-hydroxycholecalciferol (25OHD3) alone or in combination (D3 + 25OHD3).
| Treatment | N | HF1 (%) | Late embryo mortality2 (%) | Dead piping embryos3 (%) | Dead post-pipped embryos4 (%) | Dead hatchlings5 (%) |
|---|---|---|---|---|---|---|
| In ovo injection | ||||||
| Diluent6 | 12 | 92.0 | 4.85 | 1.04 | 1.19 | 0.74 |
| D37 | 12 | 92.4 | 3.55 | 0.78 | 2.13 | 1.11 |
| 25OHD38 | 12 | 95.4 | 2.45 | 0.45 | 1.36 | 0.30 |
| D3+25OHD39 | 12 | 94.0 | 3.84 | 0.88 | 0.92 | 0.17 |
| P-value | 0.077 | 0.140 | 0.729 | 0.527 | 0.119 | |
| Pooled SEM | 1.48 | 0.960 | 0.408 | 0.610 | 0.960 |
Hatchability of live embryonated eggs.
Mortality between 18 and 21 doi, prior to pip.
Mortality during the pipping process.
Mortality after the pipping process.
Mortality immediately after complete emergence of hatchlings from the shell.
Eggs injected at 18 doi with 50 μl of commercial diluent.
Eggs injected at 18 doi with 50 μl of commercial diluent containing 2.4 μg of vitamin D3.
Eggs injected at 18 doi with 50 μl of commercial diluent containing 2.4 μg of 25OHD3.
Eggs injected at 18 doi with 50 μl of commercial diluent containing 2.4 μg of D3 and 2.4 μg of 25OHD3.