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. 2021 Apr 28;100(8):101220. doi: 10.1016/j.psj.2021.101220

Table 7.

Incidence of woody breast within in ovo treatment: diluent-injected control, and diluent containing 2.4 μg of vitamin D3 (D3) or 25-hydroxycholecalciferol (25OHD3) alone or in combination (D3 + 25OHD3) and dietary treatment: commercial diet or calcium and available phosphorus restricted (ReCaP) diets at 41 and 46 d of age (doa).

Treatment N Score 0 (%) Score 1 (%) Score 2 (%) Score 3 (%) Overall score
41 doa
In ovo injection
Diluent1 60 41.8 37.6 19.7 0.9ab 0.65
D32 60 41.0 42.3 10.4 6.3a 0.75
25OHD33 60 41.8 48.5 9.6 0.1b 0.55
D3+25OHD34 60 36.7 41.0 15.8 6.4a 0.79
Diet
Commercial 120 18.6b 57.5a 19.0a 4.9 1.03a
ReCaP5 120 62.0a 27.3b 8.8b 1.9 0.34b
Pooled SEM 4.47 3.08 2.27 1.98 0.078
————————————————————P value———————————–————————
In ovo 0.870 0.495 0.197 0.042 0.117
Diet 0.001 0.001 0.024 0.193 0.001
In ovo x Diet 0.903 0.509 0.153 0.379 0.800
46 doa
In ovo injection
Diluent 60 41.8 25.6 18.3 14.3 0.86
D3 60 48.8 18.6 14.6 18.0 0.90
25OHD3 60 49.0 19.1 21.6 10.3 0.76
D3+25OHD3 60 36.8 30.5 24.2 8.4 0.84
Diet
Commercial 120 31.6b 24.5 27.5a 16.4 1.10a
ReCaP 120 56.6a 22.5 11.9b 9.0 0.59b
Pooled SEM 3.50 3.38 2.64 3.61 0.099
————————————————————P value————————————————————
In ovo 0.380 0.383 0.439 0.352 0.865
Diet 0.001 0.756 0.004 0.120 0.001
In ovo x Diet 0.440 0.629 0.151 0.579 0.758
a,b

Treatment means within the same variable column within type of treatment with no common superscripts are significantly different (P < 0.05).

1

Eggs injected at 18 d of incubation (doi) with 50 μl of commercial diluent.

2

Eggs injected at 18 doi with 50 μl of commercial diluent containing 2.4 μg of vitamin D3.

3

Eggs injected at 18 doi with 50 μl of commercial diluent containing 2.4 μg of 25OHD3.

4

Eggs injected at 18 doi with 50 μl of commercial diluent containing 2.4 μg of D3 and 2.4 μg of 25OHD3.

5

A diet restricted in Ca and available P by 20% throughout the rearing period.