Figure 12.
Enhanced basal affinity between apo PPARγ LBD and coactivator CBP-1 through incorporation of PPARγ into the LBD:LBD heterodimer with RXRα.A, schematic representation of the assay setup. B, RXRα total mutant LBD was titrated against 100 nM sGFP-PPARγ LBD, 12 nM Tb-SA, and 12 nM biotinylated CBP-1 cofactor peptide. Data are the mean ± SD; N = 3. R2 equals 98.7%. CBP-1, CREB-binding protein coactivator motif 1; LBD, ligand-binding domain; PPAR, peroxisome proliferator-activated receptor; RXR, retinoid X receptor; sGFP, super folder green fluorescent protein; Tb-SA, terbium cryptate conjugated to streptavidin.