Figure 8.

Validation of RXRα wildtype and total mutant heterodimer formation with PPARγ in a cellular setting. HEK293T cells were cotransfected with 1 or 3 ng of either Gal4-RXRα wildtype or Gal4-RXRα total mutant plasmid, and/or 25 ng VP16-PPARγ plasmid, in any case in combination with the plasmids for firefly reporter and Renilla luciferase. Control experiments without any nuclear receptor plasmid and with VP16-PPARγ alone were conducted for both experiment series (wt and mutant), respectively. Luciferase fluorescence was detected using the Dual-Glo Luciferase Assay System (Promega). Data are the mean ± SD; n = 5. PPAR, peroxisome proliferator-activated receptor; RXR, retinoid X receptor.