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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Glia. 2021 Apr 14;69(8):2037–2053. doi: 10.1002/glia.24011

FIGURE 2.

FIGURE 2

Time lapse imaging of the effect of nicotine on cultured astrocytes from Aldh1l1-tdTomato mice after mecamylamine pretreatment. (a) Quantification of the number of processes at 30-minute intervals for 12 hours at different concentrations of nicotine in astrocytes pretreated with 20 μM of mecamylamine for 30 minutes. Data are expressed in the form of mean ± SEM from 3–5 independent culture conditions. (b) Aldh1l1-tdTomato astrocytes pretreated with 20 μM of mecamylamine imaged at different time intervals in the absence of further treatment (0 hours, 6 hours, and 12 hours, scalebar = 40 μm). (c) Aldh1l1-tdTomato astrocytes pretreated with 20 μM of mecamylamine imaged at different time intervals after treatment with 0.1 μM nicotine (0 hours, 6 hours, and 12 hours, scalebar = 40 μm). (d) Aldh1l1-tdTomato astrocytes pretreated with 20 μM of mecamylamine imaged at different time intervals after treatment with 1 μM nicotine (0 hours, 6 hours, and 12 hours, scalebar = 40 μm). (e) Aldh1l1-tdTomato astrocytes pretreated with 20 μM of mecamylamine imaged at different time intervals after treatment with 10 μM nicotine (0 hours, 6 hours, and 12 hours, scalebar = 40 μm).