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. 2021 Jul 6;21:353. doi: 10.1186/s12935-021-02045-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Expression and function analysis of unreported signature genes ISG20 and SEH1L in OC. a, b Immunohistochemistry (a) and western blotting (b) analysis of ISG20 and SEH1L protein levels in paired tumor tissues and adjacent normal tissues. Scale bar = 50 μm. c qRT-PCR analysis of ISG20 and SEH1L mRNA levels in paired tumor tissues and adjacent normal tissues. *P < 0.05. d Western blotting analysis of ISG20 or SEH1L expression in Caov3 cells transfected with pLVX-ISG20-IRES-Neo, pLVX-SEH1L-IRES-Neo or empty vector. e Cell proliferation was detected by the CCK-8 assay. **P < 0.01; ***P < 0.001. f Cell migration and invasion were evaluated by Transwell assays. g Tumor growth curve. h. Xenograft tumors. Scale bar = 1 cm. i The weights of xenograft tumors. **P < 0.01; ***P < 0.001