Figure 1.

Sex difference in the density of pancreas-projecting sensory neurons Retrogradely labeled sensory neurons projecting in the pancreas and liver. Five-week-old male and female C57BL/6J mice were injected with 10 μL of PRV-152 (10⁹ pfu/mL) in the pancreas and with 10 μL PRV-614 (10⁹ pfu/mL) in the liver. Five days after injection, nodose and dorsal root ganglia were harvested for imaging and morphometric analysis. A. Schematic of the experimental design. B. Representative images of nodose ganglia derived from male (left panel) and female (right panel) mice displaying GFP, RFP and merged signals. C. Representative images of dorsal root ganglia derived from male (left panel) and female (right panel) mice displaying GFP, RFP and merged signals. D. Quantification of the percentage of GFP⁺, RFP⁺ and GFP⁺RFP⁺ double-positive cells in left nodose ganglia (LNG). E. Quantification of the percentage of GFP⁺, RFP⁺ and GFP⁺RFP⁺ double-positive cells in right nodose ganglia (RNG). F. Quantification of the percentage of GFP⁺, RFP⁺ and GFP⁺RFP⁺ double-positive cells in LNG and RNG. G. Quantification of the percentage of GFP⁺, RFP⁺ and GFP⁺RFP⁺ double-positive cells in dorsal root ganglia. Data represent mean ± SEM. Student's t-test: ∗p < 0.05 (n = 7–8).