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. 2021 Jun 11;297(1):100873. doi: 10.1016/j.jbc.2021.100873

Figure 2.

Figure 2

Increased presence of large autolysosomes in Synj1-deficient astrocytes.A, cultured astrocytes from Synj1 WT, HET, and KO brains expressing GFP-LC3. Arrows point to circular LC3 structures. B, analysis of the occurrence of circular GFP-LC3 structures in littermate astrocyte cultures presented by the stacked column plot. The number of cells analyzed from each genotype was indicated on the column. Data from three independent batches of culture. p Values are from the Mann–Whitney U test. C, WT, HET, and KO astrocytes were transfected with GFP-LC3 and immunolabeled with anti-GFP, EEA1, and Rab7 or anti-GFP, LAMP1, and p62 in separate experiments. Arrows point to circular GFP-LC3 structures, which colocalize with one of the protein markers. D, bar plot for the fraction of circular GFP-LC3 structure that colocalizes with EEA1, Rab7, LAMP1, or p62. The total numbers of GFP-LC3 structures analyzed in each category were indicated. Data from three batches of WT culture, two batches of HET culture, and three batches of KO culture. HET, heterozygous; Synj1, Synaptojanin1.