Fig. 2.

Computational scanning and experimental mutagenesis of SPT15 via Target-AID base editor. a Predicted effects of Spt15 amino acid residues. The predicted functions of amino acid residues were retrieved by artificially applying a point mutation of alanine at each position from the MutFunc database (http://www.mutfunc.com/), which has precomputed data for all possible mutations. Numbers of different variant consequences were counted and labeled on the pie chart. b The procedure of in situ mutagenizing Spt15 via base editing as well as stress tolerance genotyping and phenotyping of Spt15 mutant strains