Table 1.
Bacterial strains and plasmids.
| Strains or plasmids | Characteristics | References |
|---|---|---|
| Strains | ||
| CE08 | APEC wild-type strain, O2: K1 serotype | This study |
| ΔdctR | dctR deletion mutant in CE08 | This study |
| CΔdctR | ΔdctR with plasmid pSTV28-dctR | This study |
| DH5α | F-, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17(rk-, mk +), phoA, supE44, λ- | TIANGEN |
| Plasmids | ||
| pSTV28-Pamp | Cm, lacZ, Pamp promoter | This study |
| pSTV28-dctR | pSTV28-Pamp derivative harboring dctR gene | This study |
| pKD46 | Amp; expresses λ red recombinase | [24] |
| pKD3 | Cm, template plasmid | [24] |
| pCP20 | Cm, Amp, yeast Flp recombinase gene, FLP | [24] |