Figure 1.

Immunohistochemistry for SARS-CoV-2 Nucleoprotein (N) and spike (S) detection in the infected PFA-fixed, paraffin-embedded, sectioned COPD derived airway epithelium (in vitro)

SARS-CoV-2 N (A, shown in green) or S (B, shown in cyan) was detected by immunostaining using N- or S-specific primary antibodies and corresponding fluorescent-labeled secondary antibodies, respectively. Acetyl-alpha tubulin (A, shown in cyan) and P63 (B, shown in green) were similarly detected by immunostaining. Here, F-actin (red) and nucleus (blue) were stained with rhodamine phalloidin and DAPI, respectively. The images were taken under a Leica DMI8 modular epifluorescence microscope with a 63× oil objective. The scale bar is 20 μM.