Name | Reagent |
---|---|
1× PBS | 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.47 mM KH2PO4, pH 7.4 |
95% Ethanol | 95 mL 200 proof Ethanol and 5 mL Distilled water |
70% Ethanol | 70 mL 200 proof Ethanol and 30 mL Distilled water |
10% Goat Serum in PBS | 1 mL goat serum in 10 mL of PBS |
PBST (0.05% Tween 20) | 0.5 mL of Tween-20 in 1000 mL of PBS |