| Step | Reagent | Time |
|---|---|---|
| 1 | PBS | 5 min |
| 2 | 50% Ethanol | 20 min |
| 3 | 70% Ethanol | 20 min |
| 4 | 95% Ethanol | 20 min |
| 5 | 95% Ethanol | 20 min |
| 6 | 100% Ethanol | 10 min |
| 7 | 100% Ethanol | 10 min |
| 8 | Histo-Clear I | 20 min |
| 9 | Histo-Clear II | 20 min |
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