. 2021 Mar 9;9(2):229–247. doi: 10.1177/2050640620967898

TABLE 3.

Validated standardised conditions for processing of FMT suspensions and treatment of patients with recurrent Clostridioides difficile infection

Processing of FMT suspensions
Amount of faeces	50 g ^a
Processing	Aerobic or anaerobic
Diluent	NaCl 0.9%
Cryoprotectant	Glycerol 10%
End volume	50–60 g: 200 cc
	25–30 g: 100 cc
Storage	−80°C, maximum 2 years
Timeframe between collection and storage	<6 h (rapid processing preferred)
Treatment of patients
FMT preparation	Frozen stool banked preparation
Pretreatment of patient	4–10 Days vancomycin 125–250 mg qid (or fidaxomicin 200 mg bid)
	Stop >24 h before FMT
	Bowel lavage with macrogol on day before FMT (Not necessary in case of capsule administration) ^b
	Colonoscopy
	Nasoduonal tube (infusion 10–25 cc/minute)
	Capsules, if established protocol

Abbreviations: CDI, Clostridioides difficile infection; FMT, faecal microbiota transplantation.

^{^a}

Suspensions made of 25‐g donor faeces may even suffice, but require validation. ²⁵ , ²⁶ , ²⁷

^{^b}

For upper gastrointestinal delivery for treatment of CDI, bowel lavage could be limited to 50% of the advised dose for colonoscopy cleansing.