Figure 1.

Myc is required for Pten-loss mediated leukemogenesis and for splenic T cell homeostasis

(A) Survival curves of Pten^del, Myc^del and Myc^delPten^del mice.

(B) Quantitative PCR for Myc mRNA expression in thymus (Th) or spleen (Sp) from Control mice, Myc^delPten^del mice (disease-free and leukemic) and leukemic Pten^del mice. Transcripts levels were normalized to ABL. The analysis was performed in duplicate. Error bars represent means with standard deviation (SD).

(C) Representative FACS contour plots showing CD4 and CD8 expression on thymocytes and splenocytes from the indicated genotypes. Percentages of cells in depicted gates are indicated.

(D) Percentages of CD4 and CD8 lymphocytes in spleens.

(E) Percentages of eYFP positive CD4 and CD8 lymphocytes.

(A, B, D, and E) Numbers of mice that were analyzed are indicated. (D and E) Each dot represents a distinct mouse. Error bars represent means with SD. Statistical significant differences were assessed using Mann-Whitney test: ∗p < 0.05; ∗∗∗∗p < 0.0001.