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. 2021 Jun 1;32(6):1339–1354. doi: 10.1681/ASN.2020010031

Figure 7.

Figure 7.

Downregulation of AQP2 in DicerAQP2Cre+ mice is associated with altered association of epigenetic factors with the AQP2 promoter. ChIP assays performed on IM tissue from control (dots) or DicerAQP2Cre+ (squares) mice using anti-Phf2 and anti-Kdm5c antibodies. (A) The location of the used primers (AQP2Ch2F and AQP2Ch2R) for the AQP2 gene promoter region and for a region distal from the AQP2 promoter (AQP2NCF and AQP2NCR; as a negative control). (B) The AQP2 promoter region (pAQP2) in DicerAQP2Cre+ mice had a tendency for less Phf2 interaction, and significantly more Kdm5c interaction, relative to the AQP2 promoter from control mice. This enhanced recruitment of Kdm5c is predicted to result in a closed chromatin conformation, corroborated by a significant displacement of RNA polymerase II from the AQP2 promoter region of DicerAQP2Cre+ mice. No significant binding was detected in the region away from the promoter (pAQP2NC). All data are expressed as mean±SD; two-way ANOVA has been used to compare the groups. n:3+3. *P<0.05 for comparison of control (Ctr) versus DicerAQP2Cre+ mice; # P<0.05 for comparison versus group-related IgG.