Figure 4.

CRB2 interacts with Nephrin. (A) Scheme: CRB2 and Nephrin constructs used for pull-down assays. (B) GFP-trap pull downs: full-length (fl) CRB2 wild type binds to untagged fl Nephrin, whereas CRB2 lacking the ECD (CRB2 ΔECD) or both ECD and ICD (ΔECD + ΔICD) failed to bind. PALS1 that interacts with the ICD of CRB2 was precipitated by fl and CRB2 ΔECD (n≥3). (C) Vice versa, only fl Nephrin-GFP but not Nephrin lacking the ECD was able to bind to untagged CRB2 (fl; n=4), suggesting an interaction of CRB2 and Nephrin via the extracellular protein domains. β-TUB, β-tubulin; Δ, deletion; TMR, transmembrane region.