Figure 7.

MMF prevents the accumulation of immune cells in the kidney of db/db mice. A single-cell suspension from kidney was stained with antibodies detecting CD45, CD3, F4/80, CD80, and CD206. (A) Total immune cells (CD45+), (B) lymphocytes (CD45+ CD3+), (C) macrophages (CD45+ F4/80+), and (D) the macrophage CD80/CD206 ratio were measured by flow cytometry. Data are expressed as dot plots. Horizontal bars represent the average percentage of positive cells as mean±SD (n=4–5 per group). *P<0.05, **P<0.01, ***P<0.001 by one-way ANOVA. SSC, side scatter; Veh, vehicle.