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. 2021 May 3;32(5):1210–1226. doi: 10.1681/ASN.2020081142

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Figure 7. — Characterization of CD9⁺ and CD63⁺ particles. (A) Pie chart showing CD9⁺ and CD63⁺ distribution of particles as measured by EVQuant in second void morning spot urines (n=6). (B) The 200 K pellet was divided and subjected to either anti-CD9 or anti-CD63 antibodies. The magnetic beads were added to separate antibody-bound (precipitate) from nonbound particles (supernatant). (C) Immunoblots of NHE3 and NaPi-IIa (proximal tubule marker), NKCC2 (thick ascending limb marker), NCC (distal convoluted tubule marker), and AQP2 (collecting duct marker) in particles precipitated from 200 K pellets (n=3 subjects) by either CD9- or CD63-antibody coated magnetic beads. See also Supplemental Figure 8 for the three additional subjects.