Figure 4.

Comparison of stress resistance, intracellular cAMP levels, and mRNA of G protein signaling components between WT and ΔAoRgs mutants. A. Colony morphologies of the WT and ΔAoRgs mutants after incubation on TG medium containing NaCl and H₂O₂. B. RGI values of the fungal strains incubated on TG medium containing 0.1–0.3 M NaCl for 7 days. C. RGI values of the fungal strains incubated on TG medium containing 5–15 mM H₂O₂ for 7 days. D. Intracellular cAMP levels in the hyphae were measured after the WT and ΔAoRgs mutants were cultured in PD broth for 3, 5, and 7 d. E. The relative transcript levels of genes encoding proteins involved in G protein signaling in the WT and ΔAoFlbA mutant. The WT and ΔAoFlbA mutant were incubated in PD broth at 28°C for 7 days. Mycelia were collected and induced by nematodes for 12, 24, 36, and 48 h. Error bars: standard deviation, asterisk: significant difference between the ΔAoRgs mutants and the WT strain (Tukey’s HSD, P < 0.05)