Figure 2.
Loss of non-essential members of RSC chromatin-remodeling complex affects nuclear shape in S. cerevisiae.
Live cell imaging from an unsynchronized culture of the indicated strains transformed with plasmids expressing GFP-Esc1 (nuclear membrane marker) and mRFP-Nop1 [nucleolar marker) was done to examine the nuclear and nucleolar morphology asdescribed in 41. DAPI was used to stain the nuclear DNA. The fraction of cells showing abnormal nuclear shape in each of the mutants was quantified from ~200 cells from 2 independent experiments and is shown in the bar graph. Scale-2 µm