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. 2021 Jun 22;12:678667. doi: 10.3389/fgene.2021.678667

FIGURE 1.

FIGURE 1

Effects of STAT3 overexpression on FST gene promoter activity. (A) Conservation analysis of STAT3 binding sites in FST promoter among various species, STAT3 binding sites are announced by capital letters and their locations are relative to the first nucleotide (A, + 1) of the initiation codon (ATG) of the FST gene. (B) Western blot recognition of the STAT3 expression vector (pCMV-Myc-STAT3). Lane 1: the lysate of the cells transfected with pCMV-Myc-STAT3 (89 kDa); M: Protein markers (25 kDa-90 kDa). (C) The effect of the mutation of STAT3 binding site on FST promoter activity. (D) Effects of STAT3 on FST gene promoter activity. (E) ChIP-qPCR analysis of the binding of STAT3 to the FST promoter. (F) The agarose gel electrophoresis analysis of ChIP-qPCR products. Lane 1: The qPCR products generated from the immunoprecipitated DNA which was isolated from the cells co-transfected with pCMV-Myc-STAT3 and pGL3-FST(–980/–340); Lane 2: The qPCR products generated from the immunoprecipitated DNA which was isolated from the cells co-transfected with pCMV-Myc and pGL3-FST(–980/–340). All data are representative of three independent experiments and display as the mean ± SEM. For each figure layer, statistical significance was indicated by *P < 0.05, **P < 0.01, and different letters above error bars indicated a statistical significance (P < 0.05).