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. 2021 Jun 22;12:696635. doi: 10.3389/fphar.2021.696635

FIGURE 1.

FIGURE 1

JNK and p38 MAPK mediate high glucose (HG)-induced fibrogenic protein upregulation in vitro (A) NRCFs were confirmed by vimentin and DAPI staining (original magnification × 400). Scale bars, 50 μm. Data were obtained from three independent experiments (B and C) NRCFs transfected with or without JNK or p38 MAPK siRNA were evaluated for the protein expression of JNK and p38 MAPK by western blotting (D) NRCFs were exposed to HG for 0–48 h and JNK/p38 MAPK phosphorylation and total JNK/p38 MAPK expression were determined by western blotting (E) NRCFs were transfected with or without JNK/p38 MAPK siRNA and then exposed to HG for the indicated periods of time. The protein expression of PCNA, α-SMA, TIMP2, collagen I, collagen III, and MMP2 was determined by western blotting. Data in (B–E) are presented as mean ± SEM (n = 3). p-values in (B–D) were calculated using paired Student’s t-test. p-value in (E) was calculated using one-way ANOVA with Tukey multiple comparison test. *p < 0.05, compared to the normal group; # p < 0.05, compared to the HG group.