Table 2.
Oligonucleotides used in this study.
| Name | Sequence (5′→3′)a | Purpose |
|---|---|---|
| OHS0089 | GCTGAAGTCATGATACAGGCCAAA | 5′ AfupyrG marker_F |
| OHS0090 | ATCGTCGGGAGGTATTGTCGTCAC | 3′ AfupyrG marker_R |
| OHS0281 | ATCAGACTCAGAGTGCCGTCC | 5′ vidD DF |
| OHS0282 | GCCGAAGGAGGGGTAATCAAT | 3′ vidD DR |
| OHS0283 | GGCTTTGGCCTGTATCATGACTTCA TATCGCAAGAGCATGAATATCG | 3′ vidD with AfupyrG tail |
| OHS0284 | TTTGGTGACGACAATACCTCCCGAC GCACAACCAGACAGTACCTTGG | 5′ vidD with AfupyrG tail |
| OHS0285 | ACACATCTTCGTGCCCACCT | 5′ vidD NF |
| OHS0286 | GCGCTATTTCTGGCATAGGCG | 3′ vidD NR |
| OHS0914 | aatt GCGGCCGC CTCTTCGCACACCGAGG | 5′ vidD with promoter_NotI |
| OHS0460 | aatt GCGGCCGC GGCAGTTCGCTTTCGCAG | 3′ vidD with NotI |
| OHS0449 | CCACCGAAGAGGATGCCATA | 5′ vidD RT_F |
| OHS0450 | AGATCCACTTGCCGAGAGAG | 3′ vidD RT_R |
| OHS0576 | GGTTGAAGTCGTCGGTTGAG | 5′ tpsA RT_F |
| OHS0577 | TGGAAACCGATGAGGTCACA | 3′ tpsA RT_R |
| OHS0616 | CTCCTACTCGCGTCACTTCT | 5′ orlA RT_F |
| OHS0617 | AGGAAAGACATCCACAGCCA | 3′ orlA RT_R |
aTail sequences are shown in italics. Restriction enzyme sites are in bold.