Figure 6.

TAX treatment reduces alcohol-induced apoptosis of hepatocytes. (A) Liver cells stained with immunofluorescence probes of Caspase-3. (B) The expression of protein Bax and Bcl-2 were measured, and β-actin protein level was used as a loading control. (C, D) Quantification of relative protein expression was performed by densitometric analysis. (E) Relative Caspase-3 intensity, representative quantification of immunofluorescence images at 200×, 4,6-Diamidino-2-phenylindole (DAPI) was used as a nuclear counterstain. (F) Heat map analysis of of Bax and Bcl-2 protein relative expression levels. Data are mean ± SD. n = 10 per group. **p < 0.01, *p < 0.05 vs. normal group; ##p < 0.01, #p < 0.05 vs. alcohol group.