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. 2021 Jul 5;59(1):902–909. doi: 10.1080/13880209.2021.1944220

Figure 2.

Figure 2.

PTPRM and phosphorylation of STAT3 levels were associated with the proliferation of GBM cells. A172 and U-87 MG cells were transfected with oe-PTPRM or sh-PTPRM and their negative controls (NC). (A) The mRNA level of PTPRM was quantified by qRT-PCR. (B) The protein levels of PTPRM and p-STAT3 were detected by western blot. (C) A172, U-87 MG cells were treated with sh-PTPRM or sh-NC or stattic (STAT3 inhibitor). The colony formation assay was used to detect and compare the cell cloning formation. **p < 0.01 vs. sh-NC. ***p < 0.001 vs. sh-NC or oe-NC. #p < 0.05 vs. sh-PTPRM. ##p < 0.01 vs. sh-PTPRM.