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| Enzyme | aSpecificity | Host Organism | gDNA source |
|---|---|---|---|
| BspHIV | CGCCC | Acinetobacter species H. | NEB 394 |
| CinI | GAACT | Carnobacterium inhibens | DSM 13024 |
| DthLIII | CACC | Desulfurobacterium thermolithotrophum | DSM 11699 |
| Eco86II | GAGCC | Escherichia coli NCTC86 | Dr. Swaine L. Chen |
| FnoB1III | CGCC | Fervidobacterium nodosum Rt17-B1 | DSM 5306 |
| PleII | CACCGC | Pseudomonas lemoignei | NEB 418 |
aThe cytosine base methylated in the recognition motif is underlined.
