Table 2. Modification detection by SMRT sequencing.
| (dam GATC m6A reported for E. coli clones expressing dam MTase) | ||||
|---|---|---|---|---|
| Enzyme | MTase construct | am4C % detected | Coverage | dam m6A % |
| BspHIV | CGCCC | |||
| Native organism | 98.5 | 181x | na | |
| M.BspHIV-pRRS | 97.6 | 90x | 99.9 | |
| CinI | GAACT | |||
| Native organism | 98.4 | 146x | na | |
| M.CinI-pRRS | 25.3 | 82x | 99.9 | |
| DthLIII | CACC | |||
| Native organism | 85.5 | 550x | na | |
| M.DthLIII-pRRS | 0 | 93x | 99.9 | |
| M.DthLIII+R.DthLIII-pRRS | 90.6 | 2954x | 100 | |
| Eco86II | GAGCC | |||
| Native organism | 92.3 | 77x | 99.6 | |
| M.Eco86II-pRRS | 98.9 | 76x | 99.9 | |
| FnoB1III | CGCC | |||
| Native organism | 47 | not reported | na | |
| M.FnoB1III-pRRS | 0 | 81x | 99.9 | |
| M.FnoB1III+R.FnoB1III-pRRS | 63.7 | 163x | 99.9 | |
| PleII | CACCGC | |||
| Native organism | 99.9 | 158x | na | |
| M.PleII-pRRS | 73.9 | 86x | 99.9 | |
| M.PleII-pRRS CACCNC "star" | 69.3 | 86x | 99.9 | |
a"m4C percent detected" is percent of m4C motifs called as modified using the standard PacBio methylome analysis with default settings. Coverage is per strand for the motif. dam m6A methylation calls are shown as a control for systems expressed in E. coli carrying the dam methyltransferase.