Fig 8. Affective pain develops in rat whisker pad following inoculation with replication conditional VZV ORF9cDHFR under both growth-permissive and nonpermissive conditions.
Male Sprague-Dawley rats (n = 7/group) received inoculation into the whisker pad of 2x105 PFU TRPE-associated pOka (blue circle), VZV ORF9cDHFR in buffer with 500 nM TMP (green triangle) or without TMP (purple triangle), or uninfected TRPE cell equivalent without TMP (red square). Hypersensitivity was measured at the times indicated by days (d) post-infection above each graph using a PEAP method detailed in methods. Time spent in the dark side (y-axis) of the enclosures is shown after repeated stimulation with a 60g (5.88) von Frey hair every 15 seconds, assessed over 5-min bin periods for a total of 30 min (x-axis). The side of the face stimulated depended on the position of the rat’s head, with facial stimulation at the inoculated side if its head is in the dark side of the enclosure, and stimulation of the uninoculated side of the face if in the light side of the enclosure. Error bars: SEM. Statistics: Two-way ANOVA with Bonferroni multiple comparison to TRPE negative control where *p < .05 and color coded by group.