Fig 9. Footpad inoculation by VZV ORF4nDHFR induces mechanical and thermal hypersensitivity only if IE4 is stabilized under growth-permissive conditions.
Male Sprague-Dawley rats (n = 6/group) were inoculated with 2x105 PFU pOka (blue circle), VZV ORF4nDHFR with 500 nM TMP (green triangle) or without TMP (purple triangle), or uninfected TRPE equivalents (red square). (A) MA of inoculated footpad (top) vs contralateral (bottom). (B) The same group of rats were tested for TH of the inoculated footpad (top) vs contralateral, uninoculated footpad (bottom). Error bars: SD. Statistics: Two-way ANOVA with Bonferroni multiple comparison to TRPE negative control where *p < .05 and color coded by group.