Table 1. Primer design for VZV BAC recombineering.

#	Gene	Direction	Primer Sequence (5’ ➔ 3’)
1	ORF4	Fwd	AGGCAACTGCAAACACGCAATTGTCAGATATTTTGCAGCCggatccgccaccatgatcagtctgattgcggcgttagcg
		Rev	TCACAAATAGTAGACACGTCTGGGTCGGTTGGAATTGAAGCAGAGGCGCAtgctcgccgctccagaatct
2	ORF9	Fwd	CGTGTTTGGATATTTCACGACCCTATCGTTTATTTACGTAggatccgccaccatgatcagtctgattgcggcgttagcg
		Rev	CATTAGAGCGACAAAGTCTGTCACCGTCGGAAGATGCCATtgctcgccgctccagaatct
3	ORF63	Fwd	AGCCCCGCGCCGGCATGATATACCGCCCCCCCATGGCGTGatgatcagtctgattgcggc
		Rev	AAGACACGAGCCAAACCATTGTATTTATTTATAAAGActatgctcgccgctccagaatct
4	ORF70	Fwd	GTTTTGTTGTGCAGGGTTCGTCCGATTCATAACGCGACAGaaatgtgcgcggaacccctatttg
		Rev	TATCCACAACACCCCACTCCCCCACAGACAGACATCAAAActtggtctgacagttaccaatgctt

Uppercase letters in all rows denote bases that share sequence homology with VZV for directional recombination. Lowercase letters are sequences homologous to the E. coli DHFR degron insert sequence (#1, 2, 3) or ampicillin resistance cassette sequence (#4).