Table 2. Developed VZV BAC and degron containing VZV recombinants.
Gene | Protein | Function | Terminus attached | Resulting VZV |
---|---|---|---|---|
ORF4 | IE4 | regulator of mRNA export | N | replication conditional |
ORF6 | ORF6p | DNA primase | C | not viable |
ORF9 | ORF9p | tegument protein | C | replication conditional |
ORF10 | ORF10p | transcriptional activator | N or C | not replication conditional |
ORF29 | ORF29p | ssDNA binding protein | N or C | not viable |
ORF52 | ORF52p | DNA helicase | C | not viable |
ORF55 | ORF55p | DNA helicase | C | not replication conditional |
ORF61 | ORF61p | transcriptional regulator | N or C | not viable |
ORF62 | IE62 | major transcriptional activator | N or C | not replication conditional |
ORF63 | IE63 | transcriptional regulator | C | replication conditional |