Figure 5.
Local and systemic upregulation of immune-related genes involved in the metabolism or signaling pathways of NHP, SA, and camalexin upon treatment of Arabidopsis leaves with NHP. A, Relative transcript levels of indicated genes in leaves of Col-0 plants infiltrated with 1 mM NHP or H2O (treated 1° leaves, lower graph), and in distant nontreated 2° leaves (upper graph) at 24-h post-1° leaf treatment, as assessed by RT-qPCR analysis. B, Relative transcript levels of PR1 and ALD1 in treated (1°) leaves of Col-0, sid2-2, npr1-1, and sid2-2 npr1-1 plants infiltrated with NHP or H2O (left graphs), and in untreated, distant (2°) leaves of the same plants (right graphs). Transcript levels of genes are given as means ± sd of four biological replicates (n = 4) and are expressed relative to the respective H2O-control value. Asterisks denote statistically significant differences between NHP- and H2O-treatments (*P < 0.05, **P < 0.01, ***P < 0.001; two-tailed t test).