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. 2021 Mar 21;186(3):1591–1605. doi: 10.1093/plphys/kiab140

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© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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MtHDTs are homologous to AtHDT1, 2. A, Phylogenetic tree of HDT proteins. The protein sequences are obtained from Medicago truncatula (Mt), Lotus japonicus (Lj), Glycine max (Gm), Arabidopsis thaliana (At), Populus trichocarpa (Pt), and Oryza sativa (Os). Scale bar represents substitutions per site. B and C, Localization of pMtHDT2::GFP::HDT2 (B) and pMtHDT1::GFP::HDT1 (C) in longitudinal sections of Medicago root tips. Magnifications of boxed areas are shown in Supplemental Figure S1. Arrowheads indicate the boundary between root meristem and elongation zone. Scale bars = 100 µm.