Figure 6.

ABAleon2.1_Tao3s responses in Arabidopsis seedlings upon ABA and mannitol application. A, B, FLIM images and data showing substantial inductions of both cytosolic (A) and ER ABA (B) in different Arabidopsis tissues 1 h after application of 50-μM ABA. The GC/EC data of ABA_ER was not included as the signals in the leaves of ABAleon2.1_Tao3_ER were generally low and did not produce enough recordings for experimental analysis. The colored calibration bars indicate higher ABA concentrations with high τ_mT (red) and lower ABA concentrations with low τ_mT (blue). C, D, FLIM images and data reveal increases in ABA_cyto (C) and ABA_ER (D) within 1 h upon 200-mM mannitol treatment in root meristem zone and elongation/maturation zone, respectively, in wild-type Col-0 but not in the root of the mutant bg1-2. FLIM data are presented as means ± se. Statistical analysis performed using Student’s t test (*P < 0.01; ***P < 0.001; ns, not significant)