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. 2021 Apr 15;186(3):1455–1472. doi: 10.1093/plphys/kiab168

Figure 1.

Figure 1

FAP level corresponds to the amount of 7-heptadecene in C. reinhardtii strains mutated in FAP. A, CrFAP gene structure and site of cassette insertion in fap-1 knockout strain. Yellow boxes: exons; gray boxes: untranslated regions; red arrow: antibiotic resistance cassette. B, Relative content of total fatty-acid-derived HCs measured on whole cells; the only fatty acid-derived HC of C. reinhardtii is 7-heptadecene (abbreviated as C17:1 alk). Values are mean ± sd of n = 3 independent experiments for each strain. nd: not detected. C, Immunoblot of total protein extract probed with anti-FAP antibody. D, Loading control of the immunoblot (Ponceau staining). B, C, and D, WT: wild type strains; fap: FAP knockout strains; Cp-1–4: strains complemented with FAP gene (fap-1 background). In C and D, each track corresponds to the genotype situated just above in (B).