Figure 7.

Identification of lipid molecular species significantly different between C. reinhardtii WT and fap strains. Relative abundance of glycerolipids was measured by LC–MS/MS analysis of total lipid extracts of whole cells. Only glycerolipid molecular species showing significant differences between WT and fap strains are shown here (see Supplemental Figure S12 for complete results). WT, fap, and Cp: wild-type, FAP knockout and complementation strains, respectively. Lipid extracts from the three strains were loaded on a constant total fatty acid basis. Data are mean ± sd of n = 9 independent cultures (using three different strains for each of the three genotypes). MGDG34:4-1 is one of the two species of MGDG 34:1 species (which differ by C18:3 fatty acid isomers). Asterisks indicate significant differences according to a Mann–Whitney U-test at ^*P < 0.05 or ^**P < 0.01. Cells were grown in TAP medium, under 80 µmol photons m⁻² s⁻¹ in Erlenmeyers.