Figure 2.

PGDH1-silenced lines incorporate more [¹⁴C-3] serine-derived radioactivity. Plants were germinated and pre-cultivated on plates containing half-strength MS medium supplemented with 0.1-mM serine. Subsequently, plants were transferred to liquid half-strength MS medium supplemented with 0.1-mM serine and traced with 50 µCi [¹⁴C-3] serine for 48 h. After incubation, shoot and root materials were harvested separately and fractionated into DNA, proteins, polar, and lipophilic compounds. A, The incorporation of radioactive carbon derived from ¹⁴C-labeled serine into DNA, proteins, polar, and lipophilic compounds in shoot and root tissues of EV and PGDH1-silenced lines (ts-pgdh1.1 [1], ts-pgdh1.1 [2]) was determined as counts per minute using a scintillation counter. B, Based on these data, the percent distribution of radioactivity in the individual compounds was calculated. For (A) and (B), data presented are means ± sd of n = 5. Asterisks indicate significantly different values between EV and PGDH1-silenced lines by the Student’s t test (*P < 0.05).