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. 2021 Mar 22;186(3):1660–1678. doi: 10.1093/plphys/kiab142

Figure 2.

Figure 2

VaPAT1 interacts with VaIDD3. A, VaPAT1 protein structural representation in (B). B, Analysis of VaPAT1 and VaIDD3 transactivation activity. VaPAT1 (full length), D1 (N-terminal only) and D2 (C-terminal only), each fused with GAL4 DNA-BD protein were expressed in yeast. The transactivation activity was revealed through the expression of the lacZ reporter gene (β-galactosidase activity). Vectors pGBKT7 and pGBKT7-53 + pGADT7-Rec2-53 were expressed in yeast as a negative (N) and a positive (P) control, respectively. C, Y2H assay showing VaPAT1 and VaIDD3 interaction. P and N indicate the positive and negative control, respectively. D, In vivo interactions between VaPAT1 and VaIDD3 proteins by BiFC assays in N. benthamiana leaf cells. The reconstitution of YFP is shown. Scale bars, 100 μm.