The PAT1-ED revealed reduced expression of LOX3 and decreased cold tolerance. A, LTEs of 41B (WT), EV, and PAT1-ED (PAT1 mutants encoding truncated PAT1 lacking the C-terminal region, a loss-of-function mutation) lines. The error bars indicate the SD from three biological repeats and the letters above the bars indicate significant differences (P < 0.05, according to Duncan’s multiple range test). B, Expression levels of LOX3 in PAT1-ED line. The error bars indicate the SD from three biological repeats and asterisk indicates significant differences from the wild-type plants. *P < 0.01 (Student’s t test). C, JA-Ile content in callus of 41B/WT and PAT1-ED lines under normal condition (Control) and cold-treated (Cold, 4°C, 4 h), respectively. The error bars indicate the SD from three biological repeats and asterisk indicates significant differences from the wild-type plants. *P < 0.05 (Student’s t test).