Figure 5.

ACO activities in leaf extracts of grxs15 mutants. A, ACO activity of GRXS15^amiR and GRXS15 K83A line #4 compared to the respective WTs in leaf extracts from 3-week-old seedlings, measured by spectrophotometry in a coupled assay. n = 3; means ± sd. WT ACO activity was 35.3 ± 2.2 mU/mg protein for GRXS15^amiR and 50.6 ± 5.1 mU/mg for the parental line of GRXS15 K83A lines, while the activity for the mutants was 35 ± 2.4 and 46.6 ± 2.1 mU/mg, respectively. B, Protein blot analysis of ACO in total leaf extracts from individual plants under denaturing conditions (all three ACO isozymes have the same electrophoretic mobility). Twenty micrograms of total protein extract was loaded. Total protein staining on the membrane after transfer with Ponceau S served as a loading control.