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. Author manuscript; available in PMC: 2022 Feb 19.
Published in final edited form as: ACS Chem Biol. 2021 Feb 3;16(2):277–282. doi: 10.1021/acschembio.1c00026

Figure 4.

Figure 4.

Synthetic opioids and binding affinities of vaccine-generated antiserum. (A) Chemical structures of opioids recognized by polyclonal antibodies. (B) Antiserum IC50 values were determined by competitive SPR binding assays performed on a BIAcore 3000 instrument. Serial dilutions of compound were incubated with serum, then passed over a sensor chip with the corresponding hapten-BSA conjugate immobilized on the surface. Abbreviations: NLX, naloxone; NLT, naltrexone; MeD, methadone; Oxy, oxycodone; MOP, morphine.