Figure 13.
Alteration of SAGA-dependent histone marks in SCA7 cerebellum. A, Western blot analyses showing that the bulk level of monoubiquitinated histone H2B (H2Bub) is significantly increased in the cerebellum of SCA7140Q/5Q heterozygous mice (23 weeks) compared with WT, whereas the bulk level of acetylated lysine 9 of histone H3 (H3K9ac) is not affected. B, Western blot analyses showing the increased bulk level of H2Bub and the decreased bulk level of H3K9ac in the cerebellum of SCA7140Q/140Q homozygous mice (12 weeks) compared with WT littermates. Immunodetected signals were quantified in Fiji and normalized using the level of H2B and GAPDH. Data are mean ± SEM. Two-tailed Student's t test.