Figure 2.

Patchy M2 expression in higher cortical areas. A, B, Tangential section through occipital cortex of Chrm2-tdT mouse shows patchy M2 expression throughout the thickness of L1 (A) and uniform expression in L4 (B) of V1, LM, LI, POR, PORa, AUD, and RSP. Scale and orientation in B are the same as in A. C, Higher-magnification image of LM, LI, POR, and PORa shows that M2⁺ patches are elongated parallel to the major axes (green double-headed arrows) of LM, LI, and POR. D, Mean ± SEM ratio of major/minor axial lengths of M2⁺ patches along the major axis of the containing area in V1, LM, LI, and POR (mean, 63.2 patches/mouse analyzed; Tukey's test). E, Mean ± SEM spatial density of M2⁺ patches across areas (same mice and patches as in D; Tukey's test). F, Mean ± SEM M2⁺ patches per square degree of visual space, determined by multiplying spatial densities with magnification factor of respective area (Garrett et al., 2014; same mice and patches as in D; Tukey's test; *p < 0.05; **p < 0.01; ***p < 0.001).