Figure 3.

Evidence for a direct cGAS-ribosome interaction

(A) Cytosol from U2OS cells was separated by sucrose gradient sedimentation, and fractions were immunoblotted for cGAS and representative ribosome subunits (ul2 and eS24). Asterisks denote non-specific bands.

(B) Purified ribosomes were incubated with Ni-NTA agarose, Ni-NTA agarose with immobilized human recombinant cGAS-8his, or hPrimpol1-8his (control). After washing, the eluate was analyzed by SDS-PAGE and Coomassie staining.

(C) Western blot analysis of cGAS, ribosomes (both untreated and DNase-treated ribosomes), or cGAS-ribosome complex, separated by sucrose gradient sedimentation.

See also Figure S5.