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. 2021 Jun 11;10:e67321. doi: 10.7554/eLife.67321

Video 2. Imaging of a GUV having I-BAR domain-induced tubules.

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GUVs were first incubated with IRSp53 I-BAR domain (0.05 μM, unlabeled), followed by addition of HIV-1 Gag (0.3 μM, AX488 labeled, green). GUV membranes contained 0.5 mol% of BODIPY-TR-C5-ceramide (magenta). Frame interval = 0.6 s. Time in mm:ss. Scale bar = 5 μm. GUV, giant unilamellar vesicle.