Skip to main content
. 2021 Jul 6;10:e64819. doi: 10.7554/eLife.64819
Target Primer name F primer sequence R primer sequence
EGFP CAGtoQCpreI CAGGAATTGATCCGCATTCTGCAGTCGACGGTACC GAGGCCTACCGGTGCAAGTCAGATGCTCAAGGGGC
IRES QCIRES GCACCGGTAGGCCTC GACGCGTGATCAAGCTTATCATC
Cre CretoQCpostI GCTTGATCACGCGTCGCAAAGAATTCTGAGCCGCC TGGACCACTGATATCTCGAGCGGCCGCTATCACAGATCTT
IDPA QCGICidpa AGATCTGTGATAGCGGCCGCTCGAGATATCAGTGGTCCAGGCTCAATAAAGGGCAGGTAAGTATCAAGGTTAC ATGGCTCGTACTCTATAGGCTTCAGACGCGTTCGGATTTGATC
QCGICbc_insert QCGICbc AGATCTGTGATAGCGGCCGCCCCTGGCTCACAAATACCACTGAGATCT GAGCCTGGACCACTGATATCCCCCATAATTTTTGGCAGAGGGAAAAAGATCT
IDPA gblock AATAAAGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCTGTGTGTTGGTTTTTTGTGTGTTCTGGATCAAATCCGAACGCGT
QCGICbc_oligo AGATCTGTGATAGCGGCCGCCCCTGGCTCACAAATACCACTGAGATCTSWSWSWSWSWSWSWSWSWSWSWSWAGATCTTTTTCCCTCTGCCAAAAATTATGGGGGATATCAGTGGTCCAGGCTC